/** Bellows Custom Menu Styles (Customizer) **/
/* main */
.bellows.bellows-main, .bellows.bellows-main .bellows-menu-item, .bellows.bellows-main .bellows-menu-item .bellows-target { font-family:Arial; }
/* Status: Loaded from Transient */
.comments-link {
display: none;
}
.cmplz-hidden{display:none!important;}
.ctl-bullets-container {
display: block;
position: fixed;
bottom: 0;
height: 100%;
z-index: 1049;
font-weight: normal;
height: 70vh;
overflow-x: hidden;
overflow-y: auto;
margin: 15vh auto;
}
body { background-color:#3b3939 }
.header { background-color:#ffffff }
h1 { font: normal 40px/48px Arial, Helvetica, sans-serif; color:; }
h2 { font: normal 36px/43px Arial, Helvetica, sans-serif; color:#3b3939; }
h3 { font: normal 30px/30px Arial, Helvetica, sans-serif; color:#3b3939; }
h4 { font: normal 26px/31px Arial, Helvetica, sans-serif; color:#3b3939; }
h5 { font: normal 24px/29px Arial, Helvetica, sans-serif; color:#3b3939; }
h6 { font: normal 20px/25px Arial, Helvetica, sans-serif; color:#3b3939; }
body { font-weight: normal;}
.logo_h__txt, .logo_link { font: normal 40px/38px Tahoma, Geneva, sans-serif; color:#363f45; }
.sf-menu > li > a { font: normal 14px/18px Open Sans; color:#3b3939; }
.nav.footer-nav a { font: normal 12px/14px Arial, Helvetica, sans-serif; color:#ffffff; }
/* -- customizazione home page 02/2020 -- */
.posts-grid.facing-unknown li.span12 .thumbnail {
margin: 0 30px 10px 0;
}
.posts-grid.facing-unknown li .thumbnail {
margin: 0 0 10px 0;
}
.posts-grid.facing-unknown {
border-top: 0px solid #f5f5f6;
padding: 8px 0 5px;
}
.posts-grid li {
margin-bottom: 5px;
}
p.excerpt {
margin: 0px;
text-align: left;
}
div.type-page p {
margin: 0px;
}
.banner-wrap {
overflow: hidden;
margin: 0 0 100px;
padding: 0;
}
.banner-wrap:last-of-type {
overflow: hidden;
margin: 0px;
padding: 0;
}
.banner-wrap .featured-thumbnail a {
border: 0px;
}
.banner-wrap h5 {
color: #3e454c;
font: 300 20px/1em 'Open Sans', 'sans-serif';
text-transform: uppercase;
text-align: center;
}
/* --------------------------- */
/* IVAN --------------- */
@media screen and (max-width: 600px) {
.mobile-no {
visibility: hidden;
display: none;
}
.page-id-31946 #content{
padding-top: 10px !important;
}
}
@media screen and (min-width: 601px) {
.desk-no {
visibility: hidden;
display: none;
}
}
body.term-73 article header,
body.term-88 article header{
padding-bottom: 0px;
}
body.term-73 article .post_content,
body.term-88 article .post_content{
padding-bottom: 0px;
}
body.term-73 article .excerpt,
body.term-88 article .excerpt{
margin-bottom: 0px;
}
body.term-73 article .post_meta,
body.term-88 article .post_meta{
border: none;
margin-bottom: 0px;
}
body.term-73 article .post_meta .post_category, body.term-73 article .post_meta .post_comment,body.term-88 article .post_meta .post_category, body.term-88 article .post_meta .post_comment{
display: none;
}
.single-news .title-section{
margin-bottom: 0px;
}
.single-news div[data-motopress-static-file="static/static-title.php"]{
margin-bottom: 40px;
}
.slider_off{
display:none;
}
.thirty_accordions_box .accordion-group {
margin-bottom: 10px;
}
.thirty_accordions_box .accordion-heading {
background-color: #f5f5f5;
padding: 15px 0 15px;
}
.thirty_accordions_box .accordion-heading:hover {
background-color: #DCDEDF
}
.thirty_accordions_box .accordion-heading a {
color: #3b3939;
font: 200 32px/1em 'Open Sans', 'sans-serif';
text-transform: uppercase;
}
.thirty_accordions_box .accordion-heading a:hover {
text-decoration:none;
color: #5f823e;
}
@media (min-width: 1200px){
.im-6to7 .row .span2 {
width: 141px;
}
}
@media (max-width: 767px){
.im-6to7 .row .span2 {
width: 25%;
float: left;
}
}
footer .textwidget {
font: 12px/1.2em "Helvetica Neue", Helvetica, Arial, sans-serif;
}
footer .textwidget p {
margin-bottom: 5px;
}
footer .textwidget p.phone {
margin-top: 20px;
}
footer .textwidget p.envelope {
margin-top: 20px;
}
.nav__primary .sf-menu >li >a {
height: 64.546px !important;
}
.title-box.title-centered .title-box_primary,
.title-box.title-centered .title-box_secondary{
text-align: center;
}
.page-id-203 .posts-grid h5,
.page-id-2254 .posts-grid h5,
.page-id-20877 .posts-grid h5,
.page-id-21421 .posts-grid h5{
text-transform: uppercase;
font: 300 18px/1em 'Open Sans', 'sans-serif';
margin-bottom: 36px;
}
.page-id-203 .posts-grid h5 a,
.page-id-2254 .posts-grid h5 a,
.page-id-20877 .posts-grid h5 a,
.page-id-21421 .posts-grid h5 a
{
color: #3b3939;
}
.page-id-203 .posts-grid h5 a:hover,
.page-id-2254 .posts-grid h5 a:hover,
.page-id-20877 .posts-grid h5 a:hover,
.page-id-21421 .posts-grid h5 a:hover{
text-decoration: none;
color: #5f823e;
}
.title-section .title-header {
margin-top: 30px !important;
}
.news-template-default.single.single-news #page-content p,
.news-template-default.single.single-news #page-content ul li,
.news-template-default.single.single-news #page-content ol li{
font-size: 1.2em;
line-height: 1.8em;
}
/* 30 YEARS PAGES */
.thirty-home-title {
/* margin-top: -70px; */
}
.nav__primary .sf-menu >li#menu-item-23651,
.nav__primary .sf-menu >li#menu-item-23685{
background-color: #8dbf41;
border-left: 1px solid #8dbf41;
}
.nav__primary .sf-menu >li#menu-item-23651 a,
.nav__primary .sf-menu >li#menu-item-23685 a{
color: white;
}
.nav__primary .sf-menu >li#menu-item-23651.current-menu-item,
.nav__primary .sf-menu >li#menu-item-23685.current-menu-item{
background-color: #373f45;
border-left: 1px solid #373f45;
}
.nav__primary .sf-menu >li#menu-item-23651:hover,
.nav__primary .sf-menu >li#menu-item-23685:hover{
border-left: 1px solid #e5e6e6;
}
.nav__primary .sf-menu >li#menu-item-23651:hover a,
.nav__primary .sf-menu >li#menu-item-23685:hover a{
color: #373f45;
}
.nav__primary .sf-menu >li#menu-item-23651 >.sub-menu >.menu-item a:hover,
.nav__primary .sf-menu >li#menu-item-23685 >.sub-menu >.menu-item a:hover{
color: #8dbf41;
}
.nav__primary .sf-menu >li#menu-item-23651 >.sub-menu >.menu-item.current-menu-item a{
color: #8dbf41;
}
/*nuovo organigramma */
.nav__primary .sf-menu >li
{
width: 120px;
}
.nav__primary .sf-menu >li#menu-item-30192
{
width: 196px;
}
.nav__primary .sf-menu >li#menu-item-5177
{
width: 180px;
padding: 0 20px
}
.nav__primary .sf-menu >li#menu-item-21101
{
width: 132px;
}
.nav__primary .sf-menu >li#menu-item-23651{
padding: 0 20px;
}
.nav__primary .sf-menu >li#menu-item-23685{
padding: 0 8px;
}
.nav__primary .sf-menu >li#menu-item-21288{
padding: 0 8px;
}
.nav__primary .sf-menu >li#menu-item-21285{
padding: 0 10px;
}
/* 5x1000 5xMille */
.mille-banner {
margin-top: -80px;
background: silver url("https://www.crs4.it/wp-content/uploads/2022/02/SedePula.CRS4_.01-min.jpg") no-repeat scroll center;
height: 350px;
background-size: cover;
position: relative;
}
.mille-page.mille-banner {
margin-top: -31px;
}
.text-mille{
position: absolute;
bottom: 0;
left: 0;
background-color: rgba(0,0,0,0.55);
padding: 7px 20px;
margin-left: 20px;
margin-bottom: 15px
}
.text-mille h2:first-of-type{
color: white;
font-size: 2.4em;
}
.next-banner {
margin-top: -50px;
background: silver url("https://www.crs4.it/wp-content/uploads/2023/06/next-banner.jpg") no-repeat scroll right top;
height: 350px;
background-size: cover;
position: relative;
}
.covid-banner {
margin-top: -80px;
background: silver url("https://www.crs4.it/wp-content/uploads/2021/01/CRS4againstCOVID19-min-scaled.jpg") no-repeat scroll center;
height: 250px;
background-size: cover;
position: relative;
}
.covid-page.covid-banner {
margin-top: -31px;
}
.text-covid{
position: absolute;
bottom: 0;
left: 0;
background-color: rgba(0,0,0,0.35);
padding: 7px 20px;
margin-left: 20px;
margin-bottom: 15px
}
.text-covid h2:first-of-type{
color: white;
font-size: 2.8em;
}
.text-covid a{
color: #fff;
}
.text-covid:hover{
background-color: rgba(255,255,255,0.5);
}
.text-covid a:hover{
color: #000;
text-decoration: none;
}
.button-covid{
position: absolute;
bottom: 20px;
right: 20px;
}
.single-cool_timeline #content.span8 {
/* width: 1170px; */
}
.single-cool_timeline #sidebar {
padding-top: 0px
}
.post-template-default.single.single-post #sidebar,
.single-cool_timeline #sidebar {
padding-top: 0px
}
.single-cool_timeline #sidebar .menu-item-22860,
.single-cool_timeline #sidebar .menu-item-22546{
background-color: #373f45;
}
.single-cool_timeline #sidebar .menu-item-22860 a,
.single-cool_timeline #sidebar .menu-item-22546 a{
color: white;
}
.single-cool_timeline #sidebar .menu-item-22860:hover,
.single-cool_timeline #sidebar .menu-item-22546:hover{
background-color: #dcdedf;
}
.single-cool_timeline #sidebar .menu-item-22860:hover a,
.single-cool_timeline #sidebar .menu-item-22546:hover a{
color: black;
}
.single-cool_timeline #sidebar > div {
margin-top: 0px
}
#nav_menu-4 li{
width: 100%;
}
.single-cool_timeline .type-cool_timeline p {
font: 200 1.2em/1.6em 'Open Sans', 'sans-serif';
}
.single-cool_timeline .type-cool_timeline p:last-child {
margin-bottom: 80px;
}
.post-template-default.single.single-post #content img.wp-post-image {
float:left;
padding: 0px 20px 10px 0px;
}
.post-template-default .post-author,
.post-template-default .breadcrumb,
.single-cool_timeline .post-author,
.single-cool_timeline .breadcrumb{
display: none;
}
.title-box sup {
text-transform: lowercase !important;
}
.years30-small h2 {
font-size: 32px !important;
margin: 0 !important;
display: block;
}
#main_countedown_1 .element_conteiner {
min-width:70px !important;
}
#main_countedown_1 .time_left {
background-color: #B0CA21 !important;
font: inherit !important;
color: #fff !important;
font-weight: 900 !important;
font-size: 24px !important;
padding-bottom: 20%;
padding-top: 20%;
}
#main_countedown_1 .time_description {
font: inherit !important;
font-size: 12px !important;
color: #000000;
text-transform: uppercase;
font-weight: 100 !important;
}
.animation-logo {
margin: 60px auto;
}
.wpcf7 .wpcf7-form p input[type="tel"]{
margin: 0;
height: 35px;
-webkit-box-sizing: border-box;
-moz-box-sizing: border-box;
box-sizing: border-box;
}
@media (min-width: 1200px){
.row-fluid .next{
margin-left: 30px !important;
padding-right: 30px !important;
}
.row-fluid .next-textarea{
}
}
.page-id-21694 .title-section,
.page-id-21421 .title-section,
.page-id-21469 .title-section,
.page-id-21427 .title-section,
.page-id-21426 .title-section,
.page-id-21425 .title-section,
.page-id-21446 .title-section,
.page-id-21448 .title-section,
.page-id-21450 .title-section,
.page-id-21471 .title-section,
.page-id-22360 .title-section,
.page-id-22362 .title-section,
.page-id-22364 .title-section,
.page-id-22366 .title-section,
.page-id-22656 .title-section,
.page-id-21424 .title-section,
.page-id-22965 .title-section,
.page-id-22822 .title-section,
.page-id-23096 .title-section,
.page-id-21429 .title-section,
.page-id-22835 .title-section,
.page-id-22575 .title-section,
.page-id-24274 .title-section,
.page-id-24276 .title-section,
.page-id-24951 .title-section,
.page-id-24870 .title-section,
.page-id-26625 .title-section,
.page-id-26914 .title-section,
.page-id-26940 .title-section,
.page-id-27991 .title-section,
.page-id-28721 .title-section,
.page-id-29376 .title-section,
.page-id-29732 .title-section,
.page-id-29734 .title-section,
.page-id-29722 .title-section,
.page-id-29725 .title-section,
.page-id-29728 .title-section,
.page-id-29730 .title-section,
.page-id-30423 .title-section,
.page-id-30524 .title-section,
.page-id-30839 .title-section,
.page-id-30859 .title-section,
.page-id-30864 .title-section,
.page-id-30869 .title-section,
.page-id-30856 .title-section,
.page-id-30861 .title-section,
.page-id-30866 .title-section,
.page-id-31080 .title-section,
.page-id-31100 .title-section,
.page-id-31061 .title-section,
.page-id-31184 .title-section,
.page-id-28046 .title-section,
.page-id-31893 .title-section,
.page-id-31946 .title-section,
.page-id-27787 .title-section
{
display: none;
}
.page-id-21694 #content,
.page-id-21421 #content,
.page-id-21469 #content,
.page-id-21427 #content,
.page-id-21426 #content,
.page-id-21425 #content,
.page-id-21446 #content,
.page-id-21448 #content,
.page-id-21450 #content,
.page-id-21471 #content,
.page-id-22360 #content,
.page-id-22362 #content,
.page-id-22364 #content,
.page-id-22366 #content,
.page-id-22656 #content,
.page-id-21424 #content,
.page-id-22965 #content,
.page-id-22822 #content,
.page-id-23096 #content,
.page-id-21429 #content,
.page-id-22835 #content,
.page-id-22575 #content,
.page-id-24274 #content,
.page-id-24276 #content,
.page-id-24951 #content,
.page-id-24870 #content,
.page-id-26625 #content,
.page-id-26914 #content,
.page-id-27991 #content,
.page-id-26940 #content,
.page-id-28721 #content,
.page-id-29376 #content,
.page-id-29732 #content,
.page-id-29734 #content,
.page-id-29722 #content,
.page-id-29725 #content,
.page-id-29728 #content,
.page-id-29730 #content,
.page-id-30423 #content,
.page-id-30524 #content,
.page-id-30839 #content,
.page-id-30869 #content,
.page-id-30859 #content,
.page-id-30864 #content,
.page-id-30856 #content,
.page-id-30861 #content,
.page-id-30866 #content,
.page-id-31080 #content,
.page-id-31100 #content,
.page-id-28046 #content,
.page-id-31893 #content,
.page-id-31946 #content,
.page-id-27787 #content
{
padding-top: 100px;
padding-bottom: 80px;
}
.page-id-29732 #content,
.page-id-29734 #content,
.page-id-29722 #content,
.page-id-29725 #content,
.page-id-29728 #content,
.page-id-29730 #content,
.page-id-30423 #content,
.page-id-30869 #content,
.page-id-30859 #content,
.page-id-30864 #content,
.page-id-30856 #content,
.page-id-30861 #content,
.page-id-30866 #content,
.page-id-31061 #content,
.page-id-31184 #content
{
padding-top: 40px
}
.page-id-29734 #content img{
padding-bottom: 40px;
}
img.next_site.rounded {
width: 150px;
border-radius: 50%;
border: 10px solid #e3e3e3;
}
.next_site .nav-tabs {
border-bottom: 1px solid #6b9a24
}
.next_site .nav-tabs li{
text-transform: uppercase;
font-weight: 700 !important;
font: normal 16px/18px Open Sans;
}
.next_site .nav-tabs li.active a{
background-color: #8dbf41;
color: white;
border-bottom: 1px solid #6b9a24
}
.next_site .plan h3{
text-transform: none;
font-weight: 700 !important;
font: normal 16px/18px Open Sans;
background-color: #e5e6e6;
}
.next_site .plan.highlight h3{
background-color: #8dbf41;
border-color: #6b9a24;
font-weight: 900 !important;
}
.next_site .plan h4{
text-transform: none;
font-weight: 400 !important;
font: normal 14px/18px Open Sans;
color: #373f45;
background-color: #c0c0c0;
}
.next_site .plan.highlight h4{
background-color: #b1d678;
border-color: #6b9a24;
color: white
}
.next-page.covid-page-cta-button.text-center{
text-transform:none
}
.next_site .plan .plan-container .plan-features-row{
text-transform: none;
padding:8px;
font-size: 12px;
line-height: 16px !important;
}
.next_site .plan .plan-container .plan-features-row .small{
font-size: 10px;
line-height: 12px !important;
color: #888;
}
.ngs_site .ngs_top_button {
background: #f0f1f1;
padding: 9px 15px;
border: 1px solid #e5e6e6;
border-top: 8px solid #373f45;
}
.ngs_site .ngs_top_button h1{
text-transform: uppercase;
font-weight: 700 !important;
color: #3b3939;
font: normal 16px/18px Open Sans;
}
.ngs_site .ngs_top_button p {
font-weight: 400 !important;
color: #3b3939;
font: normal 12px/16px Open Sans;
}
.ngs_site .ngs_top_button a {
font-weight: 700 !important;
color: #8dbf41;
font: normal 12px/18px Open Sans;
display: block;
margin: 12px 12px 12px 0px;
}
.row.thirty-header .title-box {
margin: 5px 0 0px;
}
.row.thirty-header .title-box .title-box_primary{
color: #3b3939;
font: 200 54px/1em 'Open Sans', 'sans-serif';
}
.row.thirty-header .title-box .title-box_secondary{
color: #3b3939;
}
.row.thirty-header.thirty-home .title-box .title-box_primary{
text-align: center;
font: 200 40px/1em 'Open Sans', 'sans-serif';
margin-bottom: 50px
}
.row.thirty-header.thirty-home .title-box .title-box_secondary{
text-align: center;
}
.row.thirty-header.thirty-home img{
margin: 30px auto 30px;
}
.row.thirty-well-centered .title-box .title-box_primary{
text-align: center;
font: 200 20px/1em 'Open Sans', 'sans-serif';
margin: 20px 0;
}
.row.thirty-well-centered .title-box .title-box_secondary{
text-align: center;
font: 200 14px/1em 'Open Sans', 'sans-serif';
}
.thirty-menu,
#menu-30years,
#menu-30anni{
padding:0px;
list-style-type: none;
background-color: #f7f7f7;
border-radius: 3px;
margin: 0;
}
#menu-covid-projects-en,
#menu-covid-projects{
padding:0px;
list-style-type: none;
background-color: #f7f7f7;
border-radius: 3px;
margin: 0;
}
.thirty-menu li,
#menu-30years li,
#menu-30anni li{
background-color: #f7f7f7;
float: left;
position: relative;
display: inline-block;
border:none !important;
padding:4px 8px;
transition: all 0.3s ease 0s;
transition-property: all;
transition-duration: 0.3s;
transition-timing-function: ease;
transition-delay: 0s;
}
#menu-covid-projects-en li,
#menu-covid-projects li{
background-color: #f7f7f7;
position: relative;
display: block;
border:none !important;
padding:9px 18px;
transition: all 0.3s ease 0s;
transition-property: all;
transition-duration: 0.3s;
transition-timing-function: ease;
transition-delay: 0s;
}
.page-list.thirty-menu .page_item > a,
#menu-30years .menu-item > a,
#menu-30anni .menu-item > a{
color: black;
text-decoration: none;
text-transform: uppercase;
font: normal 12.5px/18px Open Sans
}
#menu-covid-projects-en .menu-item > a,
#menu-covid-projects .menu-item > a{
color: black;
text-decoration: none;
text-transform: uppercase;
font: bold 15px/18px Open Sans
}
.thirty-menu li:hover,
#menu-30years li:hover,
#menu-30anni li:hover,
#menu-covid-projects li:hover,
#menu-covid-projects-en li:hover{
background-color: #dcdedf;
}
.page-list.thirty-menu .page_item.current_page_item,
#menu-30years .menu-item.current-menu-item,
#menu-30anni .menu-item.current-menu-item,
#menu-covid-projects .menu-item.current-menu-item,
#menu-covid-projects-en .menu-item.current-menu-item{
background-color: #373f45;
}
.page-list.thirty-menu .page_item.current_page_item > a,
#menu-30years .menu-item.current-menu-item > a,
#menu-30anni .menu-item.current-menu-item > a,
#menu-covid-projects .menu-item.current-menu-item > a,
#menu-covid-projects-en .menu-item.current-menu-item > a{
color: #fff
}
.page-list.thirty-menu .page_item.current_page_item:hover,
#menu-30years .menu-item.current-menu-item:hover,
#menu-30anni .menu-item.current-menu-item:hover,
#menu-covid-projects .menu-item.current-menu-item:hover,
#menu-covid-projects-en .menu-item.current-menu-item:hover{
background-color: #373f45;
}
.thirty-menu li a:hover,
#menu-30years li a:hover,
#menu-30anni li a:hover,
#menu-covid-projects li a:hover,
#menu-covid-projects-en li a:hover{
text-decoration: none;
}
#menu-30years .sub-menu,
#menu-30anni .sub-menu{
display: none;
position: absolute;
left: -1px;
width: 200px;
margin-left: 1px;
margin-top: 3.6px;
background-color: #f1f1f1;
}
#menu-30years li:hover > .sub-menu,
#menu-30years li .sub-menu:hover,
#menu-30anni li:hover > .sub-menu,
#menu-30anni li .sub-menu:hover {
visibility: visible;
opacity: 1;
display: block;
}
#menu-30years li .sub-menu li,
#menu-30anni li .sub-menu li{
display: block;
clear: both;
width: 100%;
}
.row.thirty-links ul {
list-style-type: none;
margin: 0;
padding: 0;
overflow: hidden;
}
.row.thirty-links li {
float: left;
background-color: #f0f1f1;
transition: all 0.3s ease 0s;
transition-property: all;
transition-duration: 0.3s;
transition-timing-function: ease;
transition-delay: 0s;
}
.row.thirty-links .hr {
margin: 10px 0;
}
.row.thirty-links li a {
display: block;
color: #3b3939;
text-align: center;
padding: 5px 15px;
text-decoration: none;
text-transform: uppercase;
font: normal 14px/18px Open Sans;
}
.row.thirty-links li:hover {
background-color: #dcdedf;
}
.row.thirty-links li a:hover {
text-decoration: none;
}
.row.thirty-links li.active {
background-color: #373f45;
}
.row.thirty-links li.active a {
color: #fff;
}
.row.thirty-title .title-box .title-box_primary{
color: #3b3939;
font: 200 40px/1em 'Open Sans', 'sans-serif';
margin: 30px 0;
}
.cursor-zoomin {
cursor: zoom-in;
}
p.big,
ul.big li,
ol.big li,
.row.thirty-title p{
font: 300 20px/1.5em 'Open Sans', 'sans-serif';
}
p.big a {
font-weight: 400;
}
@media (max-width: 767px){
.row.thirty-header .title-box {
margin: 5px 0 0px;
}
.row.thirty-header .title-box .title-box_primary{
color: #3b3939;
font: 200 40px/1em 'Open Sans', 'sans-serif';
margin: 20px 0;
text-align: center;
}
.row.thirty-header .title-box .title-box_secondary{
color: #3b3939;
text-align: center;
}
.row.thirty-header a img.alignright{
float: none;
margin-bottom: 50px;
}
.row.thirty-links li {
float: none;
}
.row.thirty-title .title-box .title-box_primary {
font: 200 28px/1em 'Open Sans', 'sans-serif';
}
.thirty-title iframe {
margin-top: 40px;
}
}
.thirty-logos img {
/* filter: gray;
/* -webkit-filter: grayscale(1);
filter: grayscale(1);
opacity: 0.8; */
}
.thirty-logos img:hover {
/* -webkit-filter: grayscale(0);
filter: none;
opacity: 1; */
}
.thirty-logos .small-logo {
padding: 80px;
}
/* FINE 30 years*/
#loghist {
background-color: white;
border-radius: 10px;
/* padding: 10px; */
color: black;
text-align: center;
margin-top: -50px;
}
#logocrs4footer {
padding-top: 20px;
}
.mille.covid-page-cta {
background-image: url(https://www.crs4.it/wp-content/uploads/2022/03/foto-di-gruppo_©CRS4_2-small-scaled.jpeg);
background-size: cover;
background-repeat: no-repeat;
background-attachment: fixed;
}
.covid-page-cta {
background-image: url(https://www.crs4.it/wp-content/uploads/2021/01/next_crs4_-2.jpg);
background-size: cover;
background-repeat: no-repeat;
background-attachment: fixed;
}
.covid-page-cta .covid-page-filterwhite{
background-color: rgba(0,40,95,0.3);
padding: 30px;
}
.covid-page-cta1.title-box{
margin-bottom: 4px;
}
.covid-page-cta1.title-box .title-box_primary {
font: 500 26px/1em 'Open Sans', 'sans-serif';
color: white;
}
.covid-page-cta2.title-box,
.airport4all-page-cta{
}
.covid-page-cta2.title-box .title-box_primary,
.covid-page-cta2.title-box .title-box_secondary{
margin:0px 15% 12px;
font-weight: 700;
color: white;
}
.covid-page-cta a.btn{
background: #8dbf41;
}
.covid-page-news {
}
.covid-page-news > h2 {
font: 500 27.2px/1em 'Open Sans', 'sans-serif';
text-transform: uppercase;
}
.covid-page-news .owl-wrapper{
display: flex !important;
}
.covid-page-news .owl-item{
background-color: #f7f7f7;
padding: 10px;
margin-right: 15px;
width: 180px !important;
}
.covid-page-news .owl-item:hover{
text-decoration: none;
background-color: #8dbf41;
color:black;
}
.covid-page-news .owl-item .desc h5 {
margin-top: 0px;
margin-bottom: 3px;
}
.covid-page-news .owl-item .desc h5 a{
font: 700 10.8px/1em 'Open Sans', 'sans-serif';
text-transform: uppercase;
}
.covid-page-news .owl-item .desc p{
font: 300 13.6px/1.3em 'Open Sans', 'sans-serif';
}
.covid-page-title.title-box .title-box_secondary {
font: 500 27.2px/1em 'Open Sans', 'sans-serif';
margin: 30px 0px;
}
@media (max-width: 979px) and (min-width: 768px){
#slider-wrapper {
margin-top: 66px;
}
.camera_caption > div > h2{
font-size: 1.8em !important;
padding: 0 150px !important;
font-weight: 600 !important;
}
}
@media (max-width: 767px){
#slider-wrapper {
display: none;
}
}
@media (max-width: 468px) and (min-width: 300px){
.logo .logo_tagline {
float: none;
width: 100%;
margin: 1px 0 0 0;
font: 200 10px/1.2em 'Open Sans', 'sans-serif';
}
}
@media (max-width: 480px){
#lang_sel{
zoom: 0.8;
margin-bottom: -30px;
}
.logo .logo_tagline {
margin-top: 10px;
font-size: 9px
}
.nav.nav__primary.clearfix {
padding-top: 0px
}
.button-covid{
display:none;
}
.covid-banner {
margin-top: 0px;
margin-bottom: 30px;
background: silver url("https://www.crs4.it/wp-content/uploads/2021/01/CRS4againstCOVID19-min-scaled.jpg") no-repeat scroll center;
height: 160px;
background-size: cover;
position: relative;
}
.text-covid {
width: 100%;
margin: 0px 0px 8px;
text-align: center;
}
.text-covid h2:first-of-type {
font-weight: 400;
font-size: 1.3em;
text-align: center;
}
.thirty-home-title {
/*margin-top: -50px;*/
}
.logo.pull-left {
margin: 0;
padding: 0;
}
.logo img{
width: 35%;
margin-right: 10px
}
.logo p {
}
#menu-30years li:last-child,
#menu-30anni li:last-child{
margin-bottom: 40px;
}
.thirty-menu li,
#menu-30years li,
#menu-30anni li{
width: 100%;
}
#menu-30years .sub-menu,
#menu-30anni .sub-menu{
display: block;
visibility: visible;
opacity: 1;
display: block;
}
#menu-30years .sub-menu,
#menu-30anni .sub-menu{
display: block;
visibility: visible;
opacity: 1;
display: block;
position: relative;
background-color: transparent;
}
#menu-30years .sub-menu li,
#menu-30anni .sub-menu li{
display: block;
visibility: visible;
opacity: 1;
display: block;
position: relative;
background-color: transparent;
}
#menu-30years .current-menu-item .sub-menu li a,
#menu-30anni .current-menu-item .sub-menu li a{
color: white;
}
#menu-30years .sub-menu li a:before,
#menu-30anni .sub-menu li a:before{
content: "- ";
}
#menu-30years .sub-menu li:last-child,
#menu-30anni .sub-menu li:last-child{
margin-bottom: 0px;
}
.title-box .title-box_secondary {
font-size: 16px;
}
#loghist {
margin-top: 10px;
}
}
#nav_menu-3 h4:lang(it)::before{
content: "Ricerca e Sviluppo";
}
#nav_menu-3 h4:lang(en)::before{
content: "R&D Shortcuts";
}
#general_info h4:lang(it)::before{
content: "Informazioni generali";
}
#general_info h4:lang(en)::before{
content: "General Info";
}
.ctl-bullets-container{
/* display: none !important;*/
}
table.datacenter-table {
border:2px solid #666
}
table.datacenter-table th,
table.datacenter-table td{
border:1px solid #666;
padding: 8px 15px
}
table.datacenter-table th{
background-color: #7cb632;
color: white;
padding: 8px 5px
}
#wpcf7-f26913-p26914-o1 .input-btn.btn.btn-from-top,
#wpcf7-f26913-p26940-o1 .input-btn.btn.btn-from-top{
padding: 0;
}
#wpcf7-f26913-p26914-o1 .input-btn.btn.btn-from-top input,
#wpcf7-f26913-p26940-o1 .input-btn.btn.btn-from-top input{
padding: 15px 50px;
height: auto;
}
#wpcf7-f26913-p26914-o1 .input-btn.btn.btn-from-top .ajax-loader,
#wpcf7-f26913-p26940-o1 .input-btn.btn.btn-from-top .ajax-loader{
display: none;
}
#wpcf7-f26913-p26914-o1 form.sent .wpcf7-response-output,
#wpcf7-f26913-p26940-o1 form.sent .wpcf7-response-output{
border: 1px solid #46b450 !important;
background-color: #c1efc5;
color: #0d3503;
font-size: 24px;
line-height: 32px;
padding: 28px !important;
}
#wpcf7-f26913-p26914-o1 form.invalid .wpcf7-response-output,
#wpcf7-f26913-p26940-o1 form.invalid .wpcf7-response-output{
font-size: 20px;
line-height: 32px;
padding: 18px !important;
}
/********************************/
/********************************/
/* font awesome v5 Icon Footer */
.contacts p.home:before {
font-family: "Font Awesome 5 Free";
font-weight: 900;
content: "\f015" !important;
}
.contacts p.phone::before {
font-family: "Font Awesome 5 Free";
font-weight: 900;
content: "\f879" !important;
}
.contacts p.envelope:before {
font-family: "Font Awesome 5 Free";
font-weight: 900;
content: '\f0e0' !important;
}
#back-top-wrapper #back-top a span::after {
font-family: "Font Awesome 5 Free";
content: "\f35b";
font-weight: 900;
position: absolute;
left: 50%;
top: 50%;
margin: -8px 0px 0px -6px !important;
}
/*******************************/
/* fonts opensans by google */
/* open-sans-300 - latin */
@font-face {
font-family: 'Open Sans';
font-style: normal;
font-weight: 300;
src: url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-300.eot'); /* IE9 Compat Modes */
src: local(''),
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-300.eot?#iefix') format('embedded-opentype'), /* IE6-IE8 */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-300.woff2') format('woff2'), /* Super Modern Browsers */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-300.woff') format('woff'), /* Modern Browsers */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-300.ttf') format('truetype'), /* Safari, Android, iOS */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-300.svg#OpenSans') format('svg'); /* Legacy iOS */
}
/* open-sans-regular - latin */
@font-face {
font-family: 'Open Sans';
font-style: normal;
font-weight: 400;
src: url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-regular.eot'); /* IE9 Compat Modes */
src: local(''),
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-regular.eot?#iefix') format('embedded-opentype'), /* IE6-IE8 */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-regular.woff2') format('woff2'), /* Super Modern Browsers */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-regular.woff') format('woff'), /* Modern Browsers */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-regular.ttf') format('truetype'), /* Safari, Android, iOS */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-regular.svg#OpenSans') format('svg'); /* Legacy iOS */
}
/* open-sans-500 - latin */
@font-face {
font-family: 'Open Sans';
font-style: normal;
font-weight: 500;
src: url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-500.eot'); /* IE9 Compat Modes */
src: local(''),
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-500.eot?#iefix') format('embedded-opentype'), /* IE6-IE8 */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-500.woff2') format('woff2'), /* Super Modern Browsers */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-500.woff') format('woff'), /* Modern Browsers */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-500.ttf') format('truetype'), /* Safari, Android, iOS */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-500.svg#OpenSans') format('svg'); /* Legacy iOS */
}
/* open-sans-600 - latin */
@font-face {
font-family: 'Open Sans';
font-style: normal;
font-weight: 600;
src: url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-600.eot'); /* IE9 Compat Modes */
src: local(''),
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-600.eot?#iefix') format('embedded-opentype'), /* IE6-IE8 */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-600.woff2') format('woff2'), /* Super Modern Browsers */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-600.woff') format('woff'), /* Modern Browsers */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-600.ttf') format('truetype'), /* Safari, Android, iOS */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-600.svg#OpenSans') format('svg'); /* Legacy iOS */
}
/* open-sans-700 - latin */
@font-face {
font-family: 'Open Sans';
font-style: normal;
font-weight: 700;
src: url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-700.eot'); /* IE9 Compat Modes */
src: local(''),
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-700.eot?#iefix') format('embedded-opentype'), /* IE6-IE8 */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-700.woff2') format('woff2'), /* Super Modern Browsers */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-700.woff') format('woff'), /* Modern Browsers */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-700.ttf') format('truetype'), /* Safari, Android, iOS */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-700.svg#OpenSans') format('svg'); /* Legacy iOS */
}
/* open-sans-800 - latin */
@font-face {
font-family: 'Open Sans';
font-style: normal;
font-weight: 800;
src: url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-800.eot'); /* IE9 Compat Modes */
src: local(''),
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-800.eot?#iefix') format('embedded-opentype'), /* IE6-IE8 */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-800.woff2') format('woff2'), /* Super Modern Browsers */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-800.woff') format('woff'), /* Modern Browsers */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-800.ttf') format('truetype'), /* Safari, Android, iOS */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-800.svg#OpenSans') format('svg'); /* Legacy iOS */
}
/* open-sans-300italic - latin */
@font-face {
font-family: 'Open Sans';
font-style: italic;
font-weight: 300;
src: url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-300italic.eot'); /* IE9 Compat Modes */
src: local(''),
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-300italic.eot?#iefix') format('embedded-opentype'), /* IE6-IE8 */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-300italic.woff2') format('woff2'), /* Super Modern Browsers */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-300italic.woff') format('woff'), /* Modern Browsers */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-300italic.ttf') format('truetype'), /* Safari, Android, iOS */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-300italic.svg#OpenSans') format('svg'); /* Legacy iOS */
}
/* open-sans-italic - latin */
@font-face {
font-family: 'Open Sans';
font-style: italic;
font-weight: 400;
src: url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-italic.eot'); /* IE9 Compat Modes */
src: local(''),
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-italic.eot?#iefix') format('embedded-opentype'), /* IE6-IE8 */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-italic.woff2') format('woff2'), /* Super Modern Browsers */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-italic.woff') format('woff'), /* Modern Browsers */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-italic.ttf') format('truetype'), /* Safari, Android, iOS */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-italic.svg#OpenSans') format('svg'); /* Legacy iOS */
}
/* open-sans-500italic - latin */
@font-face {
font-family: 'Open Sans';
font-style: italic;
font-weight: 500;
src: url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-500italic.eot'); /* IE9 Compat Modes */
src: local(''),
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-500italic.eot?#iefix') format('embedded-opentype'), /* IE6-IE8 */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-500italic.woff2') format('woff2'), /* Super Modern Browsers */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-500italic.woff') format('woff'), /* Modern Browsers */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-500italic.ttf') format('truetype'), /* Safari, Android, iOS */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-500italic.svg#OpenSans') format('svg'); /* Legacy iOS */
}
/* open-sans-600italic - latin */
@font-face {
font-family: 'Open Sans';
font-style: italic;
font-weight: 600;
src: url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-600italic.eot'); /* IE9 Compat Modes */
src: local(''),
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-600italic.eot?#iefix') format('embedded-opentype'), /* IE6-IE8 */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-600italic.woff2') format('woff2'), /* Super Modern Browsers */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-600italic.woff') format('woff'), /* Modern Browsers */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-600italic.ttf') format('truetype'), /* Safari, Android, iOS */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-600italic.svg#OpenSans') format('svg'); /* Legacy iOS */
}
/* open-sans-700italic - latin */
@font-face {
font-family: 'Open Sans';
font-style: italic;
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src: local(''),
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-700italic.eot?#iefix') format('embedded-opentype'), /* IE6-IE8 */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-700italic.woff2') format('woff2'), /* Super Modern Browsers */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-700italic.woff') format('woff'), /* Modern Browsers */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-700italic.ttf') format('truetype'), /* Safari, Android, iOS */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-700italic.svg#OpenSans') format('svg'); /* Legacy iOS */
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/* open-sans-800italic - latin */
@font-face {
font-family: 'Open Sans';
font-style: italic;
font-weight: 800;
src: url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-800italic.eot'); /* IE9 Compat Modes */
src: local(''),
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-800italic.eot?#iefix') format('embedded-opentype'), /* IE6-IE8 */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-800italic.woff2') format('woff2'), /* Super Modern Browsers */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-800italic.woff') format('woff'), /* Modern Browsers */
url('/wp-content/themes/theme49466/fonts/open-sans-v29-latin/open-sans-v29-latin-800italic.ttf') format('truetype'), /* Safari, Android, iOS */
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/* CARLO */
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div.project-data { padding-top: 47px;
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div.people-index .reply a:hover { color: black;
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.underlined {
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.authors {
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.space5{
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ul.nooz-list > li > time {
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display: block;
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ul.nooz-list > li {
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@media (max-width: 767px) {
#people-index-container [class*="span"] {
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#people-index-container .span12 {
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width: 95%;
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@media (max-width: 420px) {
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.users-block-list {
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#people-index-container [class*="span"] {
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#people-index-container [class*="span"] {
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#ecl-notice {
border-top: 2px dashed yellowgreen;
}
/* ******************************************* */
/* PERSONALIZZAZIONI AD PERSONAM */
div.pub-tab-user-luigi-filippini,
div.proj-tab-user-luigi-filippini {
display: none;
}
/* *************************************** */
/* PERSONALIZZAZIONE GUESTBOOK */
#gwolle_gb_write_button .btn-default:hover {
background-color: brown;
border-color: brown;
}
/* ********************************* */
/* se menu su due righe bisogna abbassare i titoli */
.title-section {
margin: 40px 0;
padding: 30px 0 3px;
position: relative;
}
Hospitals and IRCCS
NGS solutions to enhance streamline operations: discover the benefits of partnering with us!
Apply for partnership now »
Research & Development
We work on research projects, providing support in all phases of the process.
Discover our services »
COMPANIES
Tailored NGS Solutions for your business (agri-food, zoological, biotechnological, ...)
Contact us »
Next Generation Sequencing Core
NEXT is a modern NGS core facility, consisting of a Laboratory, which allows high high throughput, l ow cost, production-scale sequencing experiments , combined with a data-intensive computational infrastructure (8000+ cores HPC Cluster with 4PB storage). An infrastructure, unique in Italy , designed to provides specialized capabilities for tracking all-data procedures and biodata provenance , managing complex analysis workflows and results.
NEXT has recently acquired new instruments , including Illumina 's most advanced sequencer, the NovaSeq X Plus .
For any information write to next@crs4.it
Contact form
Services on NovaSeq X Plus Library preparation + sequencing + bioinformatics analysis + storage
RNA Seq
(~ 100 M Paired-end Reads)
TruSeq Stranded Total RNA Library Prep Gold
Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus
TruSeq Stranded mRNA Library Prep
Illumina Stranded mRNA Prep, Ligation
Whole Genome Sequencing Human | Animal | Plant | Microbial
WGS (~ 120 Gb = Coverage 30X)
Illumina DNA LP (M) Tagmentation
KAPA HyperPlus kit
IDT UD Index
The Library Preparation protocols can be tailored according to the specific requirements.
Whole Exome Sequencing Human | Animal | Plant | Microbial
WES (~ 15 Gb = Coverage 100X)
Agilent SureSelect Human All ExonThe Library Preparation protocols can be tailored according to the specific requirements.
miRNA Seq
(~ 10 M Single Reads)
QIAseq miRNA Library Kit
UDI
UMI
More Services
Metagenomics
On MiSeq
Metagenomic analysis for 16S rRNA gene
Microbial Genome Resequencing,Custom Panels and Protocols
On NovaSeq X Plus | On MiSeq
The Library Preparation protocols for WGS, WES, and Transcriptome can be tailored to meet specific requirements.
High resolution HLA Typing up to the sixth digit
For custom panels and specific protocols , please contact us.
Sequencing only
On NovaSeq X Plus - flow cell 10B:
2 × 50 /100 /150 bp; ~1 Tb / ~2 Tb / ~3 Tb
On MiSeq - MiSeq Reagent Kit:
v3 (150/600-cycles); ~23 MCluster; ~3,5/~14 Gb
v2 (300/500-cycles); ~13 MCluster; ~4,5/~8 Gb
v2 Micro (300-cycles); ~4 MCluster; ~1 Gb
v2 Nano (300/500-cycles); ~1 MCluster; ~300/~500 Mb
Tailored services
Lab or Remote
We can offer personalized services, consultancy , and advanced training , both on-site at our laboratory and remotely. Please contact us .
Next Generation Sequencing
Call for partner: The NGS platform is available to the scientific community for research project collaborations Contact us next@crs4.it
OUR NGS LABORATORY
Our NGS Lab has been a pioneer in the use of NGS technology, starting from 2009 with the first sequencers produced by Illumina, two Genome Analyzers. By 2010, we upgraded to three HiSeq2000 sequencers, and later to the HiSeq3000. We have 15 years of experience , dozens of different applied protocols , and have processed thousands of samples of various types and origins .Recent acquisitions
Illumina NovaSeq X Plus the latest and and most powerful sequencer, with a production capacity of 120Tb/month
Agilent Fragment Analyzer 5300 , Capillary electrophoresis (48 capillaries) for fragment analysis and quality control of nucleic acids
Tecan Infinite 200 Pro plate reader for fluorometric quantification of nucleic acids and libraries
Illumina MiSeq sequencer for metagenomics, small panels and other custom protocols
Liquid Handler Hamilton NGS STAR Liquid Handler for Library Preparation with throughputs up to 96 sample libraries processed at once
The platform is directly interconnected with the HPC resources of the CRS4 computing center , a unique infrastructure in Italy that enables large-scale sequencing projects.web-accessible database will be integrated with a LIMS (Laboratory Information Management System) and analysis pipeline management system. The platform is designed to allow facility users to require analyses, perform customized investigations, and organize, consult, and update data related to their projects.
Areas of interest
Sequencing of the human genome or a set of specific genes for the investigation of a wide range of DNA variations that can be causative or predisposing to disease, or involved in drug response modulation; analysis of the transcriptome (RNASeq) to highlight variations in gene expression; high-resolution HLA gene typing with ambiguity elimination; characterization of the microbiota to identify pathogens; characterization of bacterial and viral pathogen genomes for epidemiological studies or to understand mechanisms of drug resistance and virulence.
Information on the complete genome of multiple animal and plant species enables the selection of more productive and disease-resistant strains and their geographic traceability; analysis of environmental DNA metagenome in agri-food production chains for the detection of contaminating species (viruses, bacteria, fungi and parasites, animals and plants) or identification of pathogens that affect crop or livestock productivity; species authentication for food fraud control; detection of unauthorized GMOs in food products; molecular epidemiology investigations in cases of foodborne illness.
Technology facilitates the discovery of new pathogens (bacteria, viruses, and fungi) regardless of in vitro culture and knowledge of genotypic or phenotypic characteristics; comprehensive genome characterization of specific microorganisms, whether isolated or from a mixed sample; high-throughput screening, useful for epidemiological investigations.
This cutting-edge technology, thanks to its more sensitive and broad-spectrum analysis capabilities, allows simultaneous investigation of a considerable number and types of DNA polymorphic regions for individual identification, inference of phenotypic characteristics (iris color, hair color, skin complexion, height, age, predisposition to baldness, and facial morphology), and geographic origin. Furthermore, in cases where the sample is degraded, the analysis of the complete mitochondrial genome provides useful information for individual identification.
OUR TEAM
Roberto Cusano
BiotechnologistHead of Next Lab –
Gessica Milia
BiologistResearcher –
Rossano Atzeni
BioinformaticianResearcher –
Lidia Leoni
Director of Biosciences and Computational Infrastructures, Smart Projects and Quantum Computing
Last Publications
The double-sided of human leukocyte antigen-G molecules in type 1 autoimmune hepatitis Cusano Roberto , Giglio Sabrina
A Multigene-Panel Study Identifies Single Nucleotide Polymorphisms Associated with Prostate Cancer Risk Cusano Roberto , Sechi Leonardo
Circulating Levels of miRNAs from 320 Family in Subjects with Lipodystrophy: Disclosing Novel Signatures of the Disease Cusano Roberto , Massidda Matteo , Montanelli Lucia, Gilio Donatella, Gatti Gianluca, Giacomina Alessandro, Costa Mario, Santini Ferruccio, Maffei Margherita
Next Generation Sequencing for miRNA Detection on the Exhaled Breath Condensate: A Pilot Study Cusano Roberto , Ferrari Paolo Albino, Massidda Matteo , Fotia Giorgio , De Matteis Sara, Cocco Pierluigi
mRNA-miRNA networks identify metabolic pathways associated to the anti-tumorigenic effect of thyroid hormone on preneoplastic nodules and hepatocellular carcinoma Cusano Roberto , Kowalik Marta Anna
Genomic Snapshot of SARS-CoV-2 in Migrants Entering Through Mediterranean Sea Routes Cusano Roberto , Rubino Salvatore
Contact us
For Hospitals, IRCSS, Universities , and other research institutions interested in establishing a collaboration, please send an email to roberto.cusano@crs4.it
For inquiries, quotations and any other information, kindly fill out the form below. We will get back to you as soon as possible.
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